|The Rabbit Immunoglobulin G n/a (Catalog #MBS135512) is an ELISA Kit produced from Multiple and is intended for research purposes only. The product is available for immediate purchase.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Immunoglobulin G ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This rabbit Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total rabbit IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.
Principle of the Assay: Rabbit IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-rabbit IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit IgG. Color development is proportional to the concentration of IgG in the samples.
Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.
Samples: Serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. Rabbit Immunoglobulin G ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.