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Rabbit anti Goat IgM (Fc specific) secondary antibody product blog

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Posted on 2016-09-06 10:16:00 by mybiosource_staff
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Tags: Secondary Antibody; Rabbit anti Goat IgM (Fc specific); Rabbit anti Goat IgM (Fc specific) secondary antibody;
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The Rabbit anti Goat IgM (Fc specific) n/a (Catalog #MBS571819) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Goat IgM (Fc specific), conjugated with Biotin reacts with Goat and may cross-react with other species as described in the data sheet. MyBioSource\'s Rabbit anti Goat IgM (Fc specific) can be used in a range of immunoassay formats including, but not limited to, ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting. Researchers should empirically determine the suitability of the Rabbit anti Goat IgM (Fc specific) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in goat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the userís choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Rabbit anti Goat IgM (Fc specific) n/a

Source: Purified normal IgM isolated from pooled goat serum. Freundís complete adjuvant is used in the first step of the immunization procedure
Product: Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20įC.
Conjugation: Biotin
Product Type: Secondary Antibodies. Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong reaction with Sheep IgM has been observed.
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