|The Psp5II n/a (Catalog #MBS638342) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.
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T 5\'-Pu G^G A C C Py-3\'.
Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends: Cfr13I, CpoI, Eco47I, SanDI
Methylation Effects: Psp5II does not cut PuGG(A/T)Cm5CPy. Blocked by overlapping Dcm methylation.
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Psp5II, more than 95% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.01uM. More than 95% of these can be recut.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Psp5II.
Stability During Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.1% Triton X-100, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 80 degree C for 20min.