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PSCA recombinant protein product blog

Posted on 2014-04-05 12:45:29 by mybiosource_staff
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Tags: PSCA; Recombinant Protein; Prostate stem cell antigen (Psca); Psca recombinant protein;
The PSCA psca (Catalog #MBS2012408) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Prostate Stem Cell Antigen (PSCA) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Prostate Stem Cell Antigen (PSCA) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the PSCA psca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PSCA psca product has the following accession number(s) (GI #10720240) (NCBI Accession #O43653.1) (Uniprot Accession #O43653). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given recombinant protein. We\'ve tested the Recombinant Prostate Stem Cell Antigen (PSCA) with the following immunoassay(s):

Effective Size Range: 10kOa to 70kOa.
Protein bands: 10kOa, 14kOa, 18kOa, 22kOa, 26kOa, 33kOa, 44kOa and 70kOa.
Double intensity bands: The 26kOa, 18kOa, 10kOa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EOTA, 2% (w/v) SOS, 100mM on, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
Accurate protein sizing on SOS-polyacrylamide gels and Western blots.
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a 50S-polyacrylamide gel.
3-5(.JL per well for mini gel; 7(.JL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0mm. The loading volume should be double for 1.5mm thick gels.
The protein marker, with molecular weight shifts of :55% and minimal band broadening, are confirmed by migration in SOS-PAGE system. Electrophoresis of the Marker on a 13-18% Tris-glycine SOS-polyacrylamide gel resolves 8 individual bands.

1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of -5%.
3. These protein markers are for SOS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SOS in the storage buffer.
4. Don\'t load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13-18%) large proteins (22-70kOa) can separate, while in low percentage gels (4-8%) small proteins (14 and 10kOa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kOa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by on oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SOS from the transfer buffer. SOS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SOS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
Storage: -20C, 1 year at -20oC.
If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (On) to approximately 50mM.)

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