Pro-ANP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; PRO-ANP elisa kit; PRO-ANP; Pro Atrial Natriuretic Peptide, Pro-ANP;
The Human Pro-ANP n/a (Catalog #MBS705246) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705246 ELISA Kit recognizes Human Pro-ANP.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Pro Atrial Natriuretic Peptide, Pro-ANP ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pro-ANP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Pro-ANP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Pro-ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Pro-ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates
Detection Range: 62.5 pg/ml-4000 pg/ml
Sensitivity: 15.6 pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human Pro Atrial Natriuretic Peptide, Pro-ANP ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.