Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow Pre Alpha 2 MG elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • CCDC87 sirna blog
 • PM20D1 sirna blog
 • Ifngr1 recombinant protein blog
 • TMEM183 sirna blog
 • IFT172 shrna blog
 • anti-KLK3 antibody blog
 • HSVII antibody (IgG) elisa kit blog
 • mIL 16 recombinant protein blog
 • anti-CD192 antibody blog
 • PCNA peptide blog
 • HIST1H3A sirna blog
 • BARHL1 sirna blog
 • C10orf32 shrna blog
 • TOR3A shrna blog
 • anti-CD4 antibody blog
 • anti-SRD5A2 antibody blog
 • anti-p27/kip1 antibody blog
 • ISR1 inhibitor blog
 • CNIH2 sirna blog
 • KLF10 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Angiopoietin 2 (ANG2) ELISA Kit
 • Interleukin 2 Receptor (IL-2R) (IL2RA) Antibody
 • 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase be... Recombinant Protein
 • FGFR1 Antibody
 • polo-like kinase 1 (Drosophila) (PLK1) ELISA Kit
 • LRP8 Antibody
 • Tumor Marker CA50, TM-CA50 ELISA Kit
 • RBC Antibody
 • Hsp27 Recombinant Protein
 • Lubricin Antibody
 • Dengue 2 full length NS1 (NS1 ) Recombinant Protein
 • 72 kDa type IV collagenase (Mmp2) Recombinant Protein
 • Plasminogen Activator Inhibitor 1 (PAI-1) ELISA Kit
 downarrow more ...

Pre Alpha 2 MG elisa kit product blog

Posted on 2015-01-07 19:17:41 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Human ELISA Kit; Alpha 2 Macroglobulin precursor; Pre Alpha 2 MG elisa kit; Pre Alpha 2 MG;
The Human Pre Alpha 2 MG a2m (Catalog #MBS730542) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS730542 ELISA Kit recognizes Human (General) Pre Alpha 2 MG.

The Pre Alpha 2 MG a2m product has the following accession number(s) (GI #66932947) (NCBI Accession #NP_000005.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Alpha 2 Macroglobulin precursor ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Pre Alpha 2 MG.

Principle of the assay: PRE-?2 MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRE-?2 MG antibody and an PRE-?2 MG-HRP conjugate. The assay sample and buffer are incubated together with PRE-?2 MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRE-?2 MG concentration since PRE-?2 MG from samples and PRE-?2 MG-HRP conjugate compete for the anti-PRE-?2 MG antibody binding site. Since the number of sites is limited, as more sites are occupied by PRE-?2 MG from the sample, fewer sites are left to bind PRE-?2 MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRE-?2 MG concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100 umoL/L
Sensitivity: 1.0umoL/L. Intended Uses: This PRE-?2 MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PRE-?2 MG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human Alpha 2 Macroglobulin precursor ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions