PPARGC1A elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; PPARGC1A; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PPARGC1A elisa kit;
The Mouse PPARGC1A ppargc1a (Catalog #MBS707053) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS707053 ELISA Kit recognizes Mouse PPARGC1A.The PPARGC1A ppargc1a product has the following accession number(s) (GI #7019499) (NCBI Accession #NP_037393.1) (Uniprot Accession #Q9UBK2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPARGC1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPARGC1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPARGC1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARGC1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: < 5.86 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Connective Tissue, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Pancreas, Thyroid tissues are correlated with this protein. Cardiovascular Diseases, Diabetes Mellitus, Type 2, Disease Models, Animal, Fatty Liver, Heart Diseases, Hyperglycemia, Inflammation, Insulin Resistance, Liver Diseases, Nervous System Diseases are some of the diseases may be linked to Mouse Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC1A ELISA Kit. The following patways have been known to be associated with this gene. PPARGC1A also interacts with the following gene(s): CREBBP, EP300, ESRRA, ESRRG, HNF4A, MAPK14, NCOA1, PPARA, PPARG, SIRT1.