PPARgamma elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; PPARgamma; Peroxisome Proliferators Activated Receptor gamma; PPARgamma elisa kit;
The Rabbit PPARgamma n/a (Catalog #MBS720587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS720587 ELISA Kit recognizes Rabbit PPARgamma.The PPARgamma n/a product has the following accession number(s) (GI #147904405) (NCBI Accession #NP_001091096.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Peroxisome Proliferators Activated Receptor gamma ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This PPARgamma ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PPARgamma in the sample, this PPARgamma ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PPARgamma concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PPARgamma utilizes a multiclonal anti-PPARgamma antibody and an PPARgamma-HRP conjugate. The assay sample and buffer are incubated together with PPARgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARgamma concentration since PPARgamma from samples and PPARgamma-HRP conjugate compete for the anti-PPARgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARgamma from the sample, fewer sites are left to bind PPARgamma-HRP conjugate. Standards of known PPARgamma concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of PPARgamma. The PPARgamma concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 50-1000pg/mL
Sensitivity: 1.0pg/mL.