Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow PMN Elastase elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • ASPM recombinant protein blog
 • Nefken's Reagent reagent blog
 • anti-TAB1 antibody blog
 • FGFR1OP shrna blog
 • Plasma - Pregnancy - 3rd Trimester human fluid blog
 • Goat Anti-Rat IgM+IgG (H+L chain specific) secondary antibody blog
 • VCAM-1/CD106 elisa kit blog
 • PLP elisa kit blog
 • Fluphenazine inhibitor blog
 • mmu-miR-495-3p mirna blog
 • Surfactant and Polymer surfactant blog
 • KREMEN1 blocking peptide blog
 • HSD17B6 sirna blog
 • RORB shrna blog
 • POU3F3 blocking peptide blog
 • EDC4 sirna blog
 • RARRES1 recombinant protein blog
 • LAIR2 sirna blog
 • WASH1 sirna blog
 • Rabbit anti Monkey IgG (heavy and light chains) secondary antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • PICP Antibody
 • Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) Antibody
 • M-CSF Recombinant Protein
 • 17 ketosteroids (17 ks) ELISA Kit
 • Circadian locomoter output cycles protein kaput (CLOCK) ELISA Kit
 • Renin receptor (ATP6AP2) Antibody
 • NADPH oxidase 3 (NOX3) ELISA Kit
 • Influenza B virus Nucleoprotein (NP) Recombinant Protein
 • POTE ankyrin domain family member E (POTEE) ELISA Kit
 • Triglyceride Quantification Colorimetric-Fluorometric Kit Assay Kit
 • Transforming Growth Factor beta 2 (TGFB2) Recombinant Protein
 • sL-Selectin (SELL) ELISA Kit
 • Fibrinogen Degradation Product, FDP ELISA Kit
 • TLR7 Antibody
 downarrow more ...
separator

PMN Elastase elisa kit product blog

separator
Posted on 2013-08-20 03:51:56 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; PMN Elastase; PMN Elastase elisa kit;
separator
The Human PMN Elastase n/a (Catalog #MBS728013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS728013 ELISA Kit recognizes Human (General) PMN Elastase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human PMN Elastase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PMN Elastase.

Principle of the assay: PMN-ELA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PMN-ELA antibody and an PMN-ELA-HRP conjugate. The assay sample and buffer are incubated together with PMN-ELA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PMN-ELA concentration since PMN-ELA from samples and PMN-ELA-HRP conjugate compete for the anti-PMN-ELA antibody binding site. Since the number of sites is limited, as more sites are occupied by PMN-ELA from the sample, fewer sites are left to bind PMN-ELA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PMN-ELA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100ng/mL
Sensitivity: 1.0ng/mL. Intended Uses: This PMN-ELA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PMN-ELA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human PMN Elastase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions