|The Human PMLC n/a (Catalog #MBS281220) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281220 ELISA Kit recognizes Human (General) PMLC.
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Intended Uses: For the quantitative detection of Human Phosphorylated Myosin Light Chain (PMLC) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PMLC in Human serum, plasma. An antibody specific for PMLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMLC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMLC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Myosin light chain is a subunit of myosin. Myosin light chains are distinct and have their own properties, distinct from the heavy chains. They are not considered "myosins" but are components of the macromolecular complexes that make up the functional myosin enzymes. Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 0.78 ng/mL - 50 ng/mL
Sensitivity: 0.39 ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%. Human Phosphorylated Myosin Light Chain (PMLC) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.