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PMLC elisa kit product blog

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Posted on 2013-09-07 19:20:42 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; Phosphorylated Myosin Light Chain; PMLC elisa kit; PMLC;
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The Human PMLC n/a (Catalog #MBS281220) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281220 ELISA Kit recognizes Human (General) PMLC.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PMLC in samples. An antibody specific for PMLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPMLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMLC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMLC bound in the initial step. The color development is stopped and the intensity of the color is measured.

PMLC n/a

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Human Phosphorylated Myosin Light Chain (PMLC) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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