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PLG elisa kit product blog

Posted on 2015-06-26 19:15:09 by mybiosource_staff
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Tags: ELISA Kit; Plasminogen; PLG elisa kit; PLG;
The PLG plg (Catalog #MBS564057) is an ELISA Kit produced from Human and is intended for research purposes only. The product is available for immediate purchase.

The PLG plg product has the following accession number(s) (GI #190026) (Uniprot Accession #P00747). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Plasminogen present in samples reacts with the anti-Plasminogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-PMG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound PMG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PMG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PMG in the test sample. The quantity of PMG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Background: Plasminogen (PMG) is a glycoprotein produced by the liver. It is the precursor for plasmin, which targets fibrin in the process of dissolution of fibrin blood clots. Plasminogen is present in plasma and most extravascular fluids. The important role of plasminogen in fibrinolytic system makes it an interesting marker for various diseases.

PLG plg

Samples: Immunoperoxidase Assay for Determination of Plasminogen in Human Samples
Assay Type: Sandwich. Intended Uses: The Plasminogen test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Plasminogen in biological samples of human. Human Plasminogen ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. PLG also interacts with the following gene(s): NGF, SERPINE1. Blood, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Mammary Gland, Pancreas, Spleen tissues are correlated with this protein. The following patways have been known to be associated with this gene. Blood Coagulation Disorders, Hemorrhage, Inflammation, Liver Diseases, Lung Diseases, Neoplasms, Nervous System Diseases, PLASMINOGEN DEFICIENCY, TYPE I, Thrombosis, Vascular Diseases are some of the diseases may be linked to Human Plasminogen ELISA Kit.
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