Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow PKR elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • PRL recombinant protein blog
 • anti-IGF1 antibody blog
 • REEP2 blocking peptide blog
 • MTFR1L sirna blog
 • ETFA recombinant protein blog
 • GDF1 peptide blog
 • GPT sirna blog
 • LBX1 sirna blog
 • GALC recombinant protein blog
 • anti-Trypsin Inhibitor, Ovomucoid antibody blog
 • anti-MSTN antibody blog
 • AQP4 recombinant protein blog
 • CD53 sirna blog
 • DVD/DHVD 3 elisa kit blog
 • ESRRA sirna blog
 • CYSLTR2 sirna blog
 • GPR88 shrna blog
 • TBL1X/TBL1XR1 peptide blog
 • WFDC2 peptide blog
 • anti-WWOX antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • albumin ELISA Kit
 • Tight junction protein ZO-1 (Tjp1), partial Recombinant Protein
 • Neogenin (NEO ) ELISA Kit
 • Neuropoietin (Ctf2) Recombinant Protein
 • E2 estradiol ELISA Kit
 • COX10 homolog, cytochrome c oxidase assembly protein, heme A... Antibody
 • tRNA N6-adenosine threonylcarbamoyltransferas e (KAE1) Recombinant Protein
 • G Protein Coupled Receptor (GPCR) ELISA Kit
 • IL-6 ELISA Kit
 • Testosterone ELISA Kit
 • Chitinase Assay Kit
 • Gram Positive Bacteria Antibody
 • IgG Purified Protein
 • KDM5A Antibody
 downarrow more ...
separator

PKR elisa kit product blog

separator
Posted on 2013-10-20 04:52:01 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; PKR; Protein Kinase R (PKR); PKR elisa kit;
separator
The Human PKR n/a (Catalog #MBS2021292) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2021292 ELISA Kit recognizes Human (General) PKR.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Protein Kinase R (PKR) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) PKR.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase R (PKR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase R (PKR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase R (PKR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase R (PKR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Tissue homogenates, Cell lysates and other biological fluids
Assay Type: Sandwich
Detection Range: 0.156-10ng/mL
Sensitivity: Typically less than 0.063ng/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase R (PKR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase R (PKR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay Procedure Summary: 1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately. Protein Kinase R (PKR) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions