|The Phosphodiesterase 2 n/a (Catalog #MBS653716) is an Enzyme produced from Bovine spleen and is intended for research purposes only. The product is available for immediate purchase.
The Phosphodiesterase 2 n/a product has the following accession number(s) (GI #21223851) (NCBI Accession #NP_629630.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Spleen exonuclease (Phosphodiesterase II) hydrolyzes 3\'-phosphomononucleotides from oligonucleotides containing a 5\'-hydroxyl terminus. The enzyme\'s specificity for the 5\'-phosphate end of a polynucleotide chain after conversion to 5\'-OH by use of E. coli alkaline phosphatase makes spleen exonuclease very important in the characterization of polynucleotide chain length, base composition and identity of terminal nucleotide.
Spleen exonuclease splits off, sequentially, nucleoside 3\'-phosphates, starting at the 5\'-OH end, from ribo-or deoxyribo-oligonucleotides. RNA "core", alkali-treated DNA, poly(A), poly(U), and poly(I) are acted upon. Prior removal of the 5\'-terminal phosphate group is essential.
Control No: C15070970
Revision No: 110612
Units/mg (dry weight):: 1.46u/mg
% ADA:: 1.2%
Unit Definition:: 1 unit increases the absorbance at 260nm by 0.200 in 30 minutes at 37 degree C, pH 6.5, with an RNA Core as substrate.
Certificate of Origin: : The raw animal product (bovine spleen) used in the manufacturing of Phosphodiesterase II is derived from materials originating in North America and collected in USDA approved facilities and inspected to be free of disease.
Activity: 10 units per vial. Reconstitution: : Reconstitute with sterile ddH20.
Optimum pH: : 5.5 using succinate and phosphate buffer and an acid DNase digest; pH 6-7 with 0.1 M acetate buffer.