PG-E2 elisa kit product blog
Tags: ELISA Kit; Rabbit ELISA Kit; Prostaglandin E2, PG-E2; PG-E2 elisa kit; PG-E2;
The Rabbit PG-E2 n/a (Catalog #MBS703037) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS703037 ELISA Kit recognizes Rabbit PG-E2.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the rabbit Prostaglandin E2, PG-E2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: Prostaglandin E2 (PGE2) is one of the prostaglandins, a group of hormone-like substances that participate in a wide range of body functions such as the contraction and relaxation of smooth muscle, the dilation and constriction of blood vessels, control of blood pressure, and modulation of inflammation. PGE2 is produced by several cell types including macrophages, fibroblasts and some malignant cells and exerts its actions through 4 receptors EP1, EP2, EP3 and EP4. All these receptors are rhodopsin-type receptors with seven transmembrane-spanning domains. However each receptor is coupled to different G proteins and uses different second messenger signaling pathways. Accounting on those receptors coupled to different G proteins, PGE2 has been shown to have both pro and anti-inflammatory actions. It induces vasodilatation by activating cAMP-coupled EP2 receptors on vascular smooth muscle and increases vascular permeability. But as inflammation progresses PGE2 promotes bronchodilatation. PGE2 also inhibits T helper cells type 1 (Th1) cytokines productions such as IL-12, IFNgamma, IL-2 and seems to favor Th 2 type of immune response. Although it has also been described that PGE2decreases IL-4 and IL-5 production as well as IgE production. PGE2 is involved in several pathologies such as periodontal disease and promotes tumor-cellssurvival.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGE2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGE2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5, 5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PGE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates, Cell lysates
Detection Range: 31.25 pg/ml-2000 pg/ml
Sensitivity: 7.81 pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. rabbit Prostaglandin E2, PG-E2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.