Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow PCT elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-CD19 antibody blog
 • GRO recombinant protein blog
 • HSV 2 antigen blog
 • Urtica dioica lectin blog
 • LF elisa kit blog
 • SEL1L blocking peptide blog
 • anti-CARD15 antibody blog
 • ZNF263 blocking peptide blog
 • Morniga M lectin blog
 • anti-CD8 antibody blog
 • GPR4 shrna blog
 • Tissue, Total Protein Fetal Normal, Tonsil lysate blog
 • CYP2C8 recombinant protein blog
 • anti-CLDN3 antibody blog
 • TPRG1L shrna blog
 • FRMD8 sirna blog
 • IgM secondary antibody blog
 • NAT1 sirna blog
 • anti-GLUT1 antibody blog
 • JAKMIP3 sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • chemokine (C-C motif) ligand 25 (CCL25) ELISA Kit
 • Hermansky Pudlak Syndrome Protein 4 (HPS4) Recombinant Protein
 • SPN Recombinant Protein
 • Aquaporin-1 (AQP1) Antibody
 • NDUFS6 cDNA Clone
 • Citropin-1.1 Recombinant Protein
 • Epstein-Barr nuclear antigen 2 (EBNA2) Recombinant Protein
 • A Disintegrin And Metalloprotease 17 (ADAM17) Antibody
 • ACY1 Antibody
 • Niemann-Pick disease, type C2 (NPC2) ELISA Kit
 • Soluble CD44S (SCD44S) ELISA Kit
 • tyrosine hydroxylase (TH) ELISA Kit
 • Cherry Antibody
 • Diacyl glycerol (DAG) ELISA Kit
 downarrow more ...
separator

PCT elisa kit product blog

separator
Posted on 2014-05-26 19:01:13 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Bovine ELISA Kit; Procalcitonin; PCT elisa kit; PCT;
separator
The Bovine PCT pct (Catalog #MBS742222) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS742222 ELISA Kit recognizes Bovine (Cow, Cattle, General) PCT.

The PCT pct product has the following accession number(s) (GI #339502384) (NCBI Accession #YP_004689804.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Procalcitonin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
 Typical Testing Data/Standard Curve (for reference only) PCT.

Intended Uses: This PCTELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine PCT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCT antibody and an PCT-HRP conjugate. The assay sample and buffer are incubated together with PCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCT concentration since PCT from samples and PCT-HRP conjugate compete for the anti-PCT antibody binding site. Since the number of sites is limited, as more sites are occupied by PCT from the sample, fewer sites are left to bind PCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCT concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 0.1 ug/mL. Bovine Procalcitonin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions