|The pCMV-GFP-LC3 n/a (Catalog #MBS169054) is an Expression Vector and is intended for research purposes only. The product is available for immediate purchase.
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• Includes separate GFP control vector
Autophagy is a lysosomal degradation pathway for cytoplasmic material, which is activated during stress conditions such as amino acid starvation or viral infection. Mammalian cells use autophagy during short periods of starvation to degrade nonessential cellular components in order to liberate nutrients for vital biosynthetic reactions. Recent results have shown that autophagy also contributes to development, growth regulation and cancer, as well as longevity.
After induction by a stress signal such as amino acid starvation, the first step in autophagy is the formation of an autophagosome. A well published autophagosome marker protein, MAP LC3, was originally identified as a microtubule associated protein and named \'microtubule-associated- protein-light-chain-3\'. LC3 is a small 16-18 kDa protein that is soluble in nonstarved cells, but becomes peripherally membrane-associated during amino acid starvation. By immunoelectron microscopy, LC3 has been shown to associate to the inner and outer limiting membranes of autophagosomes, and the membrane association is mediated by a covalent conjugation to a lipid, phosphatidylethanolamine. In Western blots, two forms of LC3 are seen, LC3I and LC3II. LC3I is found in the soluble fraction, and LC3II in the pelletable membrane fraction. Both LC3I and LC3II are seen in nonstarved cells, but during autophagy induction the proportion of LC3II increases. GFP-tagged LC3 expression might be useful as an autophagy assay.
pCMV-GFP-LC3 is a simple mammalian expression vector in which human LC-3B gene is fused in frame with GFP in the expression vector (Figure 1). A GFP vector without LC3 gene is also provided as a control. Both vector contain kanamycin and neomycin resistance genes as selection markers.
Quantity: 2 vectors; each contains 100 uL of bacterial glycerol stock.