Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow pCMV-GFP-LC3 expression vector  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • KRT6A sirna blog
 • ITIH4 peptide blog
 • anti-CREB1 antibody blog
 • [D-Trp34]-Neuropeptide Y peptide blog
 • anti-CHUK antibody blog
 • BATF3 sirna blog
 • anti-ASK1 antibody blog
 • SCNN1B shrna blog
 • anti-HLA-B antibody blog
 • anti-IL-1b antibody blog
 • Tissue, Total Protein, Rabbit Adult Normal, Pancreas lysate blog
 • RG2833 inhibitor blog
 • beta2M recombinant protein blog
 • SFMC elisa kit blog
 • mmu-miR-23a-5p mirna blog
 • Indole conjugate antigen blog
 • FEZ2 shrna blog
 • KRTAP26-1 sirna blog
 • PTGFRN sirna blog
 • anti-CD45 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Leptin Triple Antagonist Recombinant Protein
 • Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA Kit
 • farnesyltransferase, CAAX box, beta (FNTB) Antibody
 • 14-3-3 protein epsilon (YWHAE) ELISA Kit
 • lipoprotein lipase (LPL) ELISA Kit
 • CFL1 Antibody
 • Epithiospecifier protein (ESP) Recombinant Protein
 • Procollagen Type I C-Terminal Propeptide (PICP) ELISA Kit
 • Hypoxia Up Regulated 1 (HYOU1) ELISA Kit
 • CD30 Antibody
 • katanin p60 (ATPase-containing) subunit A 1 (KATNA1) Antibody
 • C4BPbeta ELISA Kit
 • ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit
 • TRPC1 (Short transient receptor potential channel 1) ELISA Kit
 downarrow more ...

pCMV-GFP-LC3 expression vector product blog

Posted on 2015-10-15 05:15:15 by mybiosource_staff
Share it on ...     
Tags: Expression Vector; pCMV-GFP-LC3; pCMV-GFP-LC3 expression vector;
The pCMV-GFP-LC3 n/a (Catalog #MBS169054) is an Expression Vector and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

• Includes separate GFP control vector

Autophagy is a lysosomal degradation pathway for cytoplasmic material, which is activated during stress conditions such as amino acid starvation or viral infection. Mammalian cells use autophagy during short periods of starvation to degrade nonessential cellular components in order to liberate nutrients for vital biosynthetic reactions. Recent results have shown that autophagy also contributes to development, growth regulation and cancer, as well as longevity.

After induction by a stress signal such as amino acid starvation, the first step in autophagy is the formation of an autophagosome. A well published autophagosome marker protein, MAP LC3, was originally identified as a microtubule associated protein and named \'microtubule-associated- protein-light-chain-3\'. LC3 is a small 16-18 kDa protein that is soluble in nonstarved cells, but becomes peripherally membrane-associated during amino acid starvation. By immunoelectron microscopy, LC3 has been shown to associate to the inner and outer limiting membranes of autophagosomes, and the membrane association is mediated by a covalent conjugation to a lipid, phosphatidylethanolamine. In Western blots, two forms of LC3 are seen, LC3I and LC3II. LC3I is found in the soluble fraction, and LC3II in the pelletable membrane fraction. Both LC3I and LC3II are seen in nonstarved cells, but during autophagy induction the proportion of LC3II increases. GFP-tagged LC3 expression might be useful as an autophagy assay.

pCMV-GFP-LC3 is a simple mammalian expression vector in which human LC-3B gene is fused in frame with GFP in the expression vector (Figure 1). A GFP vector without LC3 gene is also provided as a control. Both vector contain kanamycin and neomycin resistance genes as selection markers.

pCMV-GFP-LC3 n/a

Quantity: 2 vectors; each contains 100 uL of bacterial glycerol stock.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions