PaeI restriction enzyme product blog
Tags: Restriction Enzyme; PaeI; PaeI restriction enzyme;
The PaeI n/a (Catalog #MBS638348) is a Restriction Enzyme produced from Pseudomonas aeruginosa and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below:5\'-G C A T G^C-3\'
3\'-C^G T A C G-5\'.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given restriction enzyme. We\'ve tested the PaeI (SphI) with the following immunoassay(s):
Testing Data ()
Dilution Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
Supplied With: R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ºC. Storage Buffer: 10mM potassium phosphate (pH 7.0 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 0.15% Triton X-100 and 50% glycerol.
Unit Definition: One unit is defined as the amount of PaeI required to digest 1ug of lambda DNA fragments in 1 hr at 37 degree C in 50ul assay buffer.