Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow P4HB elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • HK-1 recombinant protein blog
 • anti-TUBG1 antibody blog
 • anti-FOSB antibody blog
 • GM recombinant protein blog
 • CHST13 sirna blog
 • HAND1 sirna blog
 • ECI2 blocking peptide blog
 • anti-CD10 antibody blog
 • Plasma, Normal, CPD, Frozen plasma blog
 • ANASTE_00397 recombinant protein blog
 • Mouse Anti-Rat IgG2c (gamma 2c chain specific) secondary antibody blog
 • SIGLEC5 sirna blog
 • Ly-6C secondary antibody blog
 • CHD1L shrna blog
 • ATP5S shrna blog
 • HNRPA2B1 recombinant protein blog
 • LIMD2 sirna blog
 • PCK1 blocking peptide blog
 • Angiotensin II [Sar1, Ala8] peptide blog
 • LRRK2 blocking peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit
 • 7-Amino-3-cephem-4-carboxyl ic Acid Biochemical
 • Autolysin (GLE) Recombinant Protein
 • CCR10 Antibody
 • HIV2 p26 Antibody
 • nucleotide-binding oligomerization domain containing 1 (NOD1... ELISA Kit
 • PDK1 - PDPK1 Antibody
 • formylmethionine (fMet) ELISA Kit
 • A Kinase Anchor Protein 1 (AKAP1) ELISA Kit
 • SCTR - SR - Secretin Receptor Antibody
 • Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) Recombinant Protein
 • ABC transporter ATP-binding protein-permease VMR1 (VMR1) Recombinant Protein
 • colony stimulating factor 2 (granulocyte-macrophage) (CSF2) ELISA Kit
 • Transferrin Receptor (TFR) ELISA Kit
 downarrow more ...
separator

P4HB elisa kit product blog

separator
Posted on 2014-04-25 19:17:10 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; P4HB; prolyl 4-hydroxylase, beta polypeptide; P4HB elisa kit;
separator
The Human P4HB p4hb (Catalog #MBS702295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702295 ELISA Kit recognizes Human (General) P4HB.

The P4HB p4hb product has the following accession number(s) (GI #20070125) (NCBI Accession #NP_000909.2) (Uniprot Accession #P07237). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human protein disulfide isomerase, PDI ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) P4HB.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 0.312 ng/ml -20 ng/ml.
Sensitivity: The minimum detectable dose of human PDI is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human protein disulfide isomerase, PDI ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Bone Marrow, Connective Tissue, Embryonic Tissue, Heart, Muscle, Nerve, Umbilical Cord, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Fibrosis, Heart Diseases, Immune System Diseases, Inflammation, Myocardial Ischemia, Necrosis, Nervous System Diseases, Weight Loss are some of the diseases may be linked to Human protein disulfide isomerase, PDI ELISA Kit. The following patways have been known to be associated with this gene. P4HB also interacts with the following gene(s): CALR, COL1A1, ERO1L, ERO1LB, LEPRE1, LEPREL1, P4HA3.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions