|The Human P4HB p4hb (Catalog #MBS702295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702295 ELISA Kit recognizes Human (General) P4HB.
The P4HB p4hb product has the following accession number(s) (GI #20070125) (NCBI Accession #NP_000909.2) (Uniprot Accession #P07237). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human protein disulfide isomerase, PDI ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 0.312 ng/ml -20 ng/ml.
Sensitivity: The minimum detectable dose of human PDI is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human protein disulfide isomerase, PDI ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Connective Tissue, Embryonic Tissue, Heart, Muscle, Nerve, Umbilical Cord, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Fibrosis, Heart Diseases, Immune System Diseases, Inflammation, Myocardial Ischemia, Necrosis, Nervous System Diseases, Weight Loss are some of the diseases may be linked to Human protein disulfide isomerase, PDI ELISA Kit. P4HB also interacts with the following gene(s): CALR, COL1A1, ERO1L, ERO1LB, LEPRE1, LEPREL1, P4HA3.