P JNK/SAPK elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Phosphorylated c Jun N terminal kinases/stress activated protein kinase; P JNK/SAPK elisa kit; P JNK/SAPK;
The Human P JNK/SAPK n/a (Catalog #MBS744311) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS744311 ELISA Kit recognizes Human P JNK/SAPK.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Phosphorylated c Jun N terminal kinases/stress activated protein kinase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: P-JNK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-P-JNK antibody and an P-JNK-HRP conjugate. The assay sample and buffer are incubated together with P-JNK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P-JNK concentration since P-JNK from samples and P-JNK-HRP conjugate compete for the anti-P-JNK antibody binding site. Since the number of sites is limited, as more sites are occupied by P-JNK from the sample, fewer sites are left to bind P-JNK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P-JNK concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 250-5000pg/mL
Sensitivity: 1.0pg/mL. Intended Uses: This P-JNK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human P-JNK. This ELISA kit for research use only, not for therapeutic applications!. Human Phosphorylated c Jun N terminal kinases/stress activated protein kinase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.