|The Human oxidized lowdensity lipoprotein antibody, OLAb n/a (Catalog #MBS704155) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704155 ELISA Kit recognizes Human (General) oxidized lowdensity lipoprotein antibody, OLAb.
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(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human oxidized lowdensity lipoprotein antibody, OLAb ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with oxidized low density lipoprotein. Standards and samples are pipetted into the wells with a biotin-conjugated oxidized low density lipoprotein and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OLAb bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma.
Assay Type: Sandwich (Quantitative)
Detection Range: 47 muU/ml -3000 muU/ml.
Sensitivity: 1.75 uIU/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human oxidized lowdensity lipoprotein antibody, OLAb ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.