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oplGH recombinant protein product blog

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Posted on 2014-08-31 18:55:47 by mybiosource_staff
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Tags: Recombinant Protein; Placental Growth Hormone; oplGH recombinant protein; oplGH;
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The oplGH gh2 (Catalog #MBS400051) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.

The oplGH gh2 product has the following accession number(s) (GI #2459884) (NCBI Accession #AAB71829.1) (Uniprot Accession #P01242). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Ph e-Pro-Ala, Less than 4% as determined by gel filtration chromatogr aphy
.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including placental growth hormone are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

oplGH gh2

Solubility: It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Protein Content: GH.Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard.
Dimers and Aggregates: Less than 4% as determined by gel filtration chromatography. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of oplGH (22 K).
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 9.
Biological Activity: oplGH is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD). Embryonic Tissue, Pituitary Gland, Placenta, Vascular tissues are correlated with this protein. oplGH also interacts with the following gene(s): GHR, JAK2, PRLR, STAT5B. Silver-Russell Syndrome are some of the diseases may be linked to Ovine placental growth hormone. The following patways have been known to be associated with this gene.
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