oGH recombinant protein product blog
Tags: Growth Hormone; Recombinant Protein; oGH recombinant protein; oGH;
The oGH gh (Catalog #MBS142092) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The oGH gh product has the following accession number(s) (GI #213890) (NCBI Accession #AAA49626.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Ph e-Pro-Ala.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Growth Hormone are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized GH G119R in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.66 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Biological Activity: GH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) and binds to this ECD with affinity similar the the wild type oGH.