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ODN ligand product blog

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Posted on 2017-06-19 05:53:23 by mybiosource_staff
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Tags: Ligand; ODN; ODN ligand;
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The ODN n/a (Catalog #MBS668915) is a Ligand and is intended for research purposes only. The product is available for immediate purchase. The CpG ODN (C792), TLR9 ligand (Class C) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ODN can be used in a range of immunoassay formats including, but not limited to, Functional Assay.
A. CpG ODN C792-mediated human TLR9 activation in TLR9/NF-kBLeeporter; HEK293 cells (Figure 2). 1. Harvest TLR9/NF-kB Leeporter; HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104cells/well. 2. Incubate cells at 37 degree C in a CO2incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN C792. 4. Incubate at 37 degree C in a CO2incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. B. CpG ODN C792-mediated mouse TLR9 activation in NF-kBLeeporter; RAW 264.7 cells (Figure 3). 1. Harvest NF-kB Leeporter; RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 104cells/well. 2. Incubate cells at 37 degree C in a CO2incubator for overnight. 3. The next day, stimulate cells with various amounts of CpG ODN C792. 4. Incubate at 37 degree C in a CO2incubator for 6-16 hours. 5. Add 30-50 ul of luciferase assay reagent per well. 6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer. Researchers should empirically determine the suitability of the ODN n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given ligand. We\'ve tested the CpG ODN (C792), TLR9 ligand (Class C) with the following immunoassay(s):
Structure (Fig-1: chemical structure of CpG ODN)
Structure ODN.

Testing Data (Fig-2. Induction of human TLR9 activity by CpG ODN C792)
Testing Data ODN.

Testing Data (Fig-2. Induction of mouse TLR9 activity by CpG ODN C792)
Testing Data ODN.

Sequence: 5\'-tCGaaCGaaCGaaCGttCGaaCGttCGaat-3\' (Class C ODN C792: Bases are phosphorothioate.) Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class An ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes An and B ODNs, which can induce IFN-alpha in pDC and activation of B cells.

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