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Obestatin elisa kit product blog

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Posted on 2015-02-15 09:51:57 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; Obestatin; Obestatin elisa kit;
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The Human Obestatin n/a (Catalog #MBS703664) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS703664 ELISA Kit recognizes Human (General) Obestatin.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Obestatin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Obestatin.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for obestatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any obestatin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for obestatin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of obestatin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates
Assay Type: Sandwich
Detection Range: 4.7 pg/ml -300 pg/ml.
Sensitivity: The minimum detectable dose of human obestatin is typically less than 1.1pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human Obestatin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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