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NT-proBNP elisa kit product blog

Posted on 2014-12-16 07:16:14 by mybiosource_staff
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Tags: ELISA Kit; Monkey ELISA Kit; NT-PROBNP elisa kit; NT-PROBNP; N-terminal pro-brain natriuretic peptide, NT-proBNP;
The Monkey NT-proBNP n/a (Catalog #MBS702216) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702216 ELISA Kit recognizes Monkey (Rhesus Macaque, Rhesus Monkey, General) NT-proBNP.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Monkey N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NT-proBNP.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NT-proBNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NT-proBNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NT-proBNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NT-proBNP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma and tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 0.78 ng/ml -50 ng/ml
Sensitivity: Typically less than 0.195 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Monkey N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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