NpFR1 small molecule product blog
Tags: Small Molecule; NpFR1; NpFR1 small molecule;
The NpFR1 n/a (Catalog #MBS807939) is a Small Molecule produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given small molecule. We\'ve tested the NpFR1 with the following immunoassay(s):
Immunofluorescence (IF) (Fluorescence behavior of NpFR1 (SIH-181) in the oxidized (black) and reduced (red) forms, using 50 uM. Excitation: 405 or 488 nm (not shown). Emission: 490 - 600 nm, with peak at 545 nm.)
Testing Data (Chemical structure of NpFR1 (SIH-181), a reversible fluorescence intensity-based redox sensor. This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF) (Fluorescence emission of NpFR1 (SIH-181, 5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Immunofluorescence (IF) (Fluorescence response of NpFR1 (SIH-181) to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4). This image is from Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
Testing Data (Imaging of NpFR1 (SIH-181) in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.). The layout of this image was modified for optimal display from the original Chem. Commun., 2014, 50, 8181, and licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).)
NpFR1, or napthalimide-flavin redox sensor 1, is a novel flavin molecule that exhibits a greater than 100-fold increase in fluorescence upon oxidation, and is easily reversible. In the oxidative form, is shows a maximum absorption at 395 and 463nm, max admission at 545nm. Treatment with a mild reducing agent (including sodium thiosulfate, sodium cyanoborohydride, DTT and glutathione) gave the reduced form which exhibited 110-fold lower absorbance, and 125-fold lower emission. Re-oxidation to its original fluorescence can be achieved by air or by hydrogen peroxide.
Solubility: Soluble in DMSO
Molecular Formula: C23H21N5O4
SMILES: C1(N(C(C2=CC5=C(C3=CC=CC1=C23)N(C4=NC(NC(C4=N5)=O)=O)CCC)=O)CCCC)=O
InChI: 1S/C23H21N5O4/c1-3-5-10-28-21(30)13-8-6-7-12-16(13)14(22(28)31)11-15-18(12)27(9-4-2)19-17(24-15)20(29)26-23(3
InChI Key: C5(C2=C1C(=CC4=C(C1=CC=C2)N(C3=NC(NC(C3=N4)=O)=O)CCC)C(N5CCCC)=O)=O.