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NOS3 elisa kit product blog

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Posted on 2015-06-26 05:21:55 by mybiosource_staff
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Tags: ELISA Kit; NOS3; Dog ELISA Kit; nitric oxide synthase 3 (endothelial cell); NOS3 elisa kit;
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The Dog NOS3 nos3 (Catalog #MBS701030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS701030 ELISA Kit recognizes Dog (Canine, General) NOS3.

The NOS3 nos3 product has the following accession number(s) (GI #75051779) (NCBI Accession #Q9TUX8.1) (Uniprot Accession #Q9TUX8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Dog Endothelial nitric oxide synthase, eNOS ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NOS3.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for eNOS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any eNOS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for eNOS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of eNOS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates, Cell lysates
Assay Type: Sandwich
Detection Range: 15.6 U/ml -1000U/ml.
Sensitivity: The minimum detectable dose of dog eNOS is typically less than 3.9 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Dog Endothelial nitric oxide synthase, eNOS ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. NOS3 also interacts with the following gene(s): ADC, ARG1, ARG2, ASL, ASS1, CAV1, NOS1, NOS2, OTC.
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