Normal Tissue Blot I immunoblot product blog
Tags: Immunoblot; Normal Tissue Blot I; Normal Tissue Blot I immunoblot;
The Normal Tissue Blot I n/a (Catalog #MBS153749) is an Immunoblot and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Normal Tissue Blot I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature. Researchers should empirically determine the suitability of the Normal Tissue Blot I n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given immunoblot. We\'ve tested the Rat Normal Tissue Blot I with the following immunoassay(s):
SDS-PAGE (Coomassie blue staining of rat tissue lysates in 10% SDS-PAGE. Lanes; 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.)
The rat tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different rat tissue lysates with 15 ug of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane.The rat tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.
Species: Rat. Quantity Loaded Per Lane: 15 ug
By Sample Identification: Normal
Samples Loaded: Lanes; 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.
Note: Lysate lane order may vary; please consult accompanying shipment paperwork.