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NGF elisa kit product blog

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Posted on 2013-07-31 17:05:30 by mybiosource_staff
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Tags: ELISA Kit; Mouse ELISA Kit; NGF elisa kit; NGF; nerve growth factor (beta polypeptide);
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The Mouse NGF ngf (Catalog #MBS702384) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702384 ELISA Kit recognizes Mouse (Murine, General) NGF.

The NGF ngf product has the following accession number(s) (GI #162951831) (NCBI Accession #NP_001106168.1) (Uniprot Accession #P01139). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Nerve growth factor, NGF ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NGF.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, cell culture supernates, tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 1.56 pg/ml -100 pg/ml
Sensitivity: Typically less than 0.39 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Mouse Nerve growth factor, NGF ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. NGF also interacts with the following gene(s): MMP7, NGFR, NTRK1, PLG. Embryonic Tissue, Eye, Intestine, Lung, Muscle, Skin, Spleen tissues are correlated with this protein. Heart Diseases, Hyperalgesia, Inflammation, Necrosis, Nerve Degeneration, Nervous System Diseases, Neurobehavioral Manifestations, Neurodegenerative Diseases, Pain, Peripheral Nervous System Diseases are some of the diseases may be linked to Mouse Nerve growth factor, NGF ELISA Kit.
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