Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow NeuN elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TWISTNB blocking peptide blog
 • CSF3 shrna blog
 • ATP6AP1 sirna blog
 • CDK5 blocking peptide blog
 • AAV5-Cre control virus blog
 • IgG2a negative/isotype control blog
 • anti-CYB561D2 antibody blog
 • LH-RH peptide blog
 • anti-EGFR antibody blog
 • anti-SOX11 antibody blog
 • ELANE protein blog
 • VEGF recombinant protein blog
 • anti-OsTip3-1 antibody blog
 • GOLGA6 peptide blog
 • HLA-C elisa kit blog
 • SLC26A1 sirna blog
 • Tissue, Section Disease, Asthma, Lung tissue section blog
 • OXSM sirna blog
 • Donkey anti IgG (heavy and light chains) secondary antibody blog
 • ZFP292 shrna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • ZNF75 Antibody
 • cathepsin D (CTSD) Antibody
 • Defensin Alpha 1, Neutrophil (DEFa1) Recombinant Protein
 • GAD1 Antibody
 • beta-Catenin (CTNNB1) Antibody
 • Microtubule Associated Protein RP-EB Family, Member 1 (MAPRE... ELISA Kit
 • Retinol Binding Protein-4 (RBP4) Recombinant Protein
 • Endothelin 1 (EDN1) Antibody
 • Histone deacetylase 10 (HDAC10) Antibody
 • Intelectin 1-Omentin (ITLN1) ELISA Kit
 • L-Selectin (L-Sel) ELISA Kit
 • Apolipoprotein B48 (APO-B48) ELISA Kit
 • Tyr-3-trp-5 Monooxygenase Activation Protein (YWHAQ) Antibody
 • triggering receptor expressed on myeloid cells 2 (TREM2) ELISA Kit
 downarrow more ...
separator

NeuN elisa kit product blog

separator
Posted on 2014-02-04 16:10:37 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; NeuN; Neuronal nuclear Antigen; NeuN elisa kit;
separator
The Human NeuN n/a (Catalog #MBS7240544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7240544 ELISA Kit recognizes Human (General) NeuN.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Neuronal nuclear Antigen ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NeuN.

Intended Uses: This NeuN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NeuN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: NeuN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NeuN antibody and an NeuN-HRP conjugate. The assay sample and buffer are incubated together with NeuN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NeuN concentration since NeuN from samples and NeuN-HRP conjugate compete for the anti-NeuN antibody binding site. Since the number of sites is limited, as more sites are occupied by NeuN from the sample, fewer sites are left to bind NeuN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NeuN concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 pg/mL. Human Neuronal nuclear Antigen ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions