Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow NEFH elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • POU5F1B shrna blog
 • PCSK4 sirna blog
 • Cardiac Troponin I/T/C Complex recombinant protein blog
 • NPTX1 peptide blog
 • anti-CD4 antibody blog
 • RAN peptide blog
 • ACTH (1-4) peptide blog
 • F10 shrna blog
 • Swine anti Human IgE (Fc specific) secondary antibody blog
 • anti-URM1 antibody blog
 • CYP2C7 shrna blog
 • CYSLTR1 sirna blog
 • TIMM8A sirna blog
 • PLG protein blog
 • TNFSF4 elisa kit blog
 • PDZD4 sirna blog
 • Tissue cDNA, First Strand Adult Normal, Heart, Ventricle cdna blog
 • DAGLA blocking peptide blog
 • anti-HMGN1 antibody blog
 • Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) secondary antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • MAPK4 Antibody
 • anti-Protein G Antibody
 • Proprotein convertase subtilisin-kexin type 9 (PCSK9), NARC1... Antibody
 • fatty acid binding protein 6, ileal (FABP6) ELISA Kit
 • 60 kDa SS-A-Ro ribonucleoprotein (TROVE2) Antibody
 • OLIG1 (C Terminus) Peptide
 • ADP-ribosylation factor 4 (ARF4) Antibody
 • H-2 class II histocompatibility antigen gamma chain (Cd74) ELISA Kit
 • Ovalbumin Antibody
 • Lungkine (Cxcl15) Recombinant Protein
 • G-protein pathway suppressor 2 (GPS2) Antibody
 • Aquaporin 4 (AQP4) ELISA Kit
 • RFK Antibody
 • Hepatitis C Virus, NS5 Antibody
 downarrow more ...
separator

NEFH elisa kit product blog

separator
Posted on 2014-12-30 15:36:40 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Neurofilament, Heavy Polypeptide; NEFH elisa kit; NEFH; Pig ELISA Kit;
separator
The Pig NEFH nefh (Catalog #MBS918487) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS918487 ELISA Kit recognizes Pig (Porcine, Swine, General) NEFH.

The NEFH nefh product has the following accession number(s) (GI #128128) (NCBI Accession #P12037.1) (Uniprot Accession #P12037). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig Neurofilament heavy polypeptide, NEFH ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NEFH.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEFH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEFH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEFH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEFH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 0.78 ng/ml-50 ng/ml
Sensitivity: 0.195 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Pig Neurofilament heavy polypeptide, NEFH ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. NEFH also interacts with the following gene(s): FLNA. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions