Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow NE elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • ISCU sirna blog
 • RETSAT sirna blog
 • SDS-PAGE Gel Preparation Kit western blot related reagent blog
 • SAG elisa kit blog
 • MUP20 sirna blog
 • anti-hCG antibody blog
 • anti-ADH1C antibody blog
 • AIMP2 sirna blog
 • RERE shrna blog
 • NO elisa kit blog
 • anti-IGA4 antibody blog
 • 1,5-AG elisa kit blog
 • anti-Fn1 antibody blog
 • anti-APOA1 antibody blog
 • TMEM241 sirna blog
 • anti-Complement C3a alpha antibody blog
 • SPRED2 sirna blog
 • SH2D4A shrna blog
 • CPNE6 blocking peptide blog
 • anti-lacZ antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • interferon-inducible protein 9, IP-9 (IP-9-CXCL11) ELISA Kit
 • Pentraxin-3 (PTX-3) ELISA Kit
 • Proteoglycan 4 (PRG4) ELISA Kit
 • Fibrinogen (Fb) ELISA Kit
 • Prion Protein (PRNP ) Peptide
 • SPIN4 Antibody
 • O-6-methylguanine-DNA methyltransferase (MGMT) ELISA Kit
 • F1+2 (F1-2) ELISA Kit
 • TROPONIN C (TNNC1) Antibody
 • Apolipoprotein L6 (Apol6) Antibody
 • SLC25A51 Antibody
 • Insulin, INS ELISA Kit
 • protein tyrosine phosphatase, non-receptor type 23 (PTPN23) ELISA Kit
 • 4E-BP1 Antibody
 downarrow more ...
separator

NE elisa kit product blog

separator
Posted on 2015-06-12 14:05:46 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Hamster ELISA Kit; Neutrophil Elastase; NE elisa kit;
separator
The Hamster NE elane (Catalog #MBS044865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS044865 ELISA Kit recognizes Hamster (Chinese Hamster, CHO, General) NE.

The NE elane product has the following accession number(s) (GI #4503549) (NCBI Accession #NP_001963.1) (Uniprot Accession #P08246). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NE elane

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type: Sandwich
Detection Range: 25 ng/ml - 800 ng/ml.
Sensitivity: 5.0 ng/ml. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NE concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster NE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NE and NE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) is less than 15%.
Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]. Hamster Neutrophil Elastase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions