NADHPox elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox; NADHPox elisa kit; NADHPox;
The Human NADHPox n/a (Catalog #MBS732086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS732086 ELISA Kit recognizes Human NADHPox.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This NADHPOX GP91PHOX ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of NADHPOX GP91PHOX in the sample, this NADHPOX GP91PHOX ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus NADHPOX GP91PHOX concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of NADHPOX GP91PHOX utilizes a multiclonal anti-NADHPOX GP91PHOX antibody and an NADHPOX GP91PHOX-HRP conjugate. The assay sample and buffer are incubated together with NADHPOX GP91PHOX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NADHPOX GP91PHOX concentration since NADHPOX GP91PHOX from samples and NADHPOX GP91PHOX-HRP conjugate compete for the anti-NADHPOX GP91PHOX antibody binding site. Since the number of sites is limited, as more sites are occupied by NADHPOX GP91PHOX from the sample, fewer sites are left to bind NADHPOX GP91PHOX-HRP conjugate. Standards of known NADHPOX GP91PHOX concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of NADHPOX GP91PHOX. The NADHPOX GP91PHOX concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Human Nicotinamide Adenine Dinucleotide Phosphate Oxidase gp91phox ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.