|The MYCOPLASMA REMOVAL AGENT n/a (Catalog #MBS238289) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MYCOPLASMA REMOVAL AGENT can be used in a range of immunoassay formats including, but not limited to, Tissue Culture (TC). Researchers should empirically determine the suitability of the MYCOPLASMA REMOVAL AGENT n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given accessory reagent. We\'ve tested the MYCOPLASMA REMOVAL AGENT with the following immunoassay(s):
Testing Data #1 (The effect of 0.5ug/ml Mycoplasma Removal Agent (MBS238289) added to the culture medium of mycoplasma-infected cells before (left) and after 1 week (right))
Intended Use: MRA is very effective at removing mycoplasma from infected cell cultures. It shows strong anti-mycoplasma activity against many types of mycoplasma including Mycoplasma orale, M. arginini, M. hyorhinis and Acholeplasma laidlawii.MRA is also suitable for use after the removal of mycoplasma, to prevent recontamination of the culture with the original mycoplasma, at preventative doses.This product can also be used to prevent initial infection of cells in culture by mycoplasma. MRA is non toxic, and will not interfere with the viability or function of cells in culture. It should be emphasised that MRA should not be used as a substitute for good cell culture techniques.
Instructions For Use: MRA is very easy to use, simply requiring incubation for a week after addition to cell cultures contaminated by mycoplasma.
Indications for Use:
1. Add MRA to cell cultures contaminated by mycoplasma at a concentration of 0.5 mug/ml and incubate
for a week.
2. For media replacement or culture transfer (passage), use a medium containing MRA at this same
3. Transfer the cell cultures several times without MRA and confirm that regrowth of the contaminating
mycoplasma has not occurred.
If there is a concern about the presence of mycoplasma in serum or trypsin, MRA can be added to the
media at a concentration of 0.5 mug/ml to prevent contamination of the cell cultures exposed to these
N.B. The recommended concentration for use is 0.5 mug/ml. The MRA concentration may be raised up to 1 mug/ml only when the recommended concentration is ineffective in removing the mycoplasma.
The cytotoxicity of MRA is low and cell toxicity is rare when used at the recommended concentration. For
specific function of any cell, however, it is recommended that the retention of desired cellular characteristics
be confirmed after treatment.