mPDGF BB recombinant protein product blog
Tags: Recombinant Protein; Platelet Derived Growth Factor-BB; mPDGF BB recombinant protein; mPDGF BB;
The mPDGF BB pdgfb (Catalog #MBS142288) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The mPDGF BB pdgfb product has the following accession number(s) (GI #40538817) (NCBI Accession #NP_035187.2) (Uniprot Accession #P31240). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Platelet Derived Growth Factor-BB are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.
Biological Activity: The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml. Atherosclerosis, Disease Models, Animal, Fibrosis, Hyperplasia, Hypertension, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Necrosis are some of the diseases may be linked to Recombinant Mouse Platelet Derived Growth Factor-BB. Blood, Brain, Embryonic Tissue, Eye, Heart, Lung, Mixed, Ovary, Skin, Spleen tissues are correlated with this protein. The following patways have been known to be associated with this gene.