|The MOUSE SEROBLOCK FCR n/a (Catalog #MBS238295) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MOUSE SEROBLOCK FCR can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Researchers should empirically determine the suitability of the MOUSE SEROBLOCK FCR n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given accessory reagent. We\'ve tested the MOUSE SEROBLOCK FcR with the following immunoassay(s):
Testing Data #2 (Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MBS213590) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (MBS238296))
MBS238295 is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in high background staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with MBS238295, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MBS210943), AlexaFluor488 (MBS212682A488), AlexaFluor647 (MBS212682A647), Biotin (MBS210447) and RPE (MBS213784).
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Instructions For Use: In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-
1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of this item (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.
2) Add test antibody according to manufacturers instructions - Do not wash This item off the cells. This item is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*
3) Proceed with staining as usual.
* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. MyBioSource supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose. Immunogen: PU5 1.8 IOE7 Balb/c mouse cell line
Buffer Solution: Phosphate buffered saline
Target Species: Mouse.