Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow MOUSE SEROBLOCK FCR accessory reagent  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TNKS2 blocking peptide blog
 • FGF6 blocking peptide blog
 • CA1 sirna blog
 • anti-GSTP1 antibody blog
 • GUK1 sirna blog
 • WDR11 sirna blog
 • OR2M4 sirna blog
 • CHIR-99021 kinase inhibitor blog
 • GAPDHS sirna blog
 • anti-TCF4 antibody blog
 • Benzodiazepine (Oxazepam) antigen blog
 • LOC554318 secondary antibody blog
 • CRP native protein blog
 • SIRT1 elisa kit blog
 • MGEA5 shrna blog
 • HA recombinant protein blog
 • ABR shrna blog
 • EN2 blocking peptide blog
 • tight junction protein 1 (ZO1) elisa kit blog
 • EBV recombinant protein blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Lck (Ab-192) Antibody
 • C1QBP Antibody
 • UBR1 Antibody
 • CHST9 Antibody
 • Nesfatin ELISA Kit
 • Osteoclase Differentiation Factor (ODF) ELISA Kit
 • Activating Transcription Factor 6 (ATF6) ELISA Kit
 • Nucleoporin 98Kda (NUP98) ELISA Kit
 • Cyclooxygenase 1 (COX1) ELISA Kit
 • Glycogen Phosphorylase, Brain (PYGB) ELISA Kit
 • Nuclear Factor Kb P65 (NFKB P65) ELISA Kit
 • Mitogen Activated Protein Kinase -Activa Protein Kinase 3 (M... ELISA Kit
 • Protein Carbonyl (PC) ELISA Kit
 • Acidic Fibroblast Growth Factor 1 (AFGF-1) ELISA Kit
 downarrow more ...
separator

MOUSE SEROBLOCK FCR accessory reagent product blog

separator
Posted on 2013-07-30 18:35:55 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Accessory Reagent; MOUSE SEROBLOCK FCR; MOUSE SEROBLOCK FCR accessory reagent;
separator
The MOUSE SEROBLOCK FCR n/a (Catalog #MBS238295) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MOUSE SEROBLOCK FCR can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Researchers should empirically determine the suitability of the MOUSE SEROBLOCK FCR n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given accessory reagent. We\'ve tested the MOUSE SEROBLOCK FcR with the following immunoassay(s):
Testing Data #2 (Hamster anti Mouse TCR Gamma/DeltaRPE antibody (MBS213590) staining mouse spleen cells in the absence (blue line) and presence (red histogram) of SeroBlock FcR (MBS238296))
Testing Data #2 MOUSE SEROBLOCK FCR.

MBS238295 is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRgammaIII and FcRgammaII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in œhigh background staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with MBS238295, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MBS210943), AlexaFluor488 (MBS212682A488), AlexaFluor647 (MBS212682A647), Biotin (MBS210447) and RPE (MBS213784).

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Instructions For Use: In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of this item (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions - Do not wash This item off the cells. This item is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. MyBioSource supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose. Immunogen: PU5 1.8 IOE7 Balb/c mouse cell line
Buffer Solution: Phosphate buffered saline
Target Species: Mouse.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions