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Mouse IgG Isotype Control isotype control product blog

Posted on 2013-12-09 17:32:30 by mybiosource_staff
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Tags: Isotype Control; Mouse IgG Isotype Control; Mouse IgG Isotype Control isotype control;
The Mouse IgG Isotype Control n/a (Catalog #MBS679134) is an Isotype Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase.
Quality tested applications include:

FC 7-10

Other referenced applications include:

ICC 12
WB 13,14
IP 15,16
ChIP 17,18
Block 19,20
In vitro control 22-25
In vivo control 2,9, 21

Flow Cytometry FITC and BIOT conjugates <=1 ug/10 6cells

PE, PE/TXRD, APC, SPRD, PE/CY5.5, PE/CY7, APC/CY5.5, and APC/CY7 conjugates <=0.1 ug/10 6cells

For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL

ELISA : AP conjugate 1:500 1:5,000

Other Applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need . Researchers should empirically determine the suitability of the Mouse IgG Isotype Control n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given isotype control. We\'ve tested the Mouse IgG-LE/AF with the following immunoassay(s):
Flow Cytometry (FLISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (MBS674380) Goat Anti-Mouse IgA-UNLB (MBS674422)and Goat Anti-Mouse IgM, Human ads-UNLB (MBS674352).Serially diluted Mouse IgG-APC (MBS679132) was captured and fluorescence intensity quantified.)
Flow Cytometry Mouse IgG Isotype Control.

Source: Normal mouse serum. Characterization: To insure lot-to-lot consistency, each batch of product is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
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