Mouse IgA elisa kit product blog
Tags: ELISA Kit; Mouse IgA; Mouse IgA elisa kit;
The Mouse Mouse IgA n/a (Catalog #MBS564073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS564073 ELISA Kit recognizes Mouse Mouse IgA.The Mouse IgA n/a product has the following accession number(s) (GI #2632187). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: The IgA test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in mouse biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgA present in samples reacts with the anti-IgA antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Samples: Mouse biological samples
Assay Type: Sandwich. Mouse IgA ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. Mouse IgA also interacts with the following gene(s): FLNA.