MMP9 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; MMP-9; MMP9; MMP9 elisa kit;
The Mouse MMP9 mmp9 (Catalog #MBS175917) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175917 ELISA Kit recognizes Mouse MMP9.The MMP9 mmp9 product has the following accession number(s) (GI #341940960) (NCBI Accession #P41245.2) (Uniprot Accession #P41245). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse MMP-9 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Picokine� Mouse Mmp9 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Mmp9 with a 96-well strip plate that is pre-coated with antibody specific for Mmp9. The detection antibody is a biotinylated antibody specific for Mmp9. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Mmp9 with immunogen: Expression system for standard: CHO; Immunogen sequence: A20-P730. The kit is analytically validated with ready to use reagents. To measure Mouse Mmp9, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Mmp9 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Mouse Mmp9 in the sample.
Background/Introduction: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogenepositive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
Samples: Cell culture supernates, serum and plasma(heparin)
Detection Range: 156pg/ml-10,000pg/ml
Sensitivity: <20pg/ml.