MMP13 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; MMP13; MMP13 elisa kit; matrix metallopeptidase 13 (collagenase 3);
The Human MMP13 mmp13 (Catalog #MBS702282) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702282 ELISA Kit recognizes Human MMP13.The MMP13 mmp13 product has the following accession number(s) (GI #4505209) (NCBI Accession #NP_002418.1) (Uniprot Accession #P45452). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Matrix metalloproteinase 13, MMP-13 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, cell culture supernates, urine, tissue homogenates
Assay Type: Sandwich
Detection Range: 0.156 ng/ml-10 ng/ml
Sensitivity: 0.039 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Developmental Protein
Protein Biological Process 3: Collagen degradation. Human Matrix metalloproteinase 13, MMP-13 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Breast Neoplasms, Disease Models, Animal, Fibrosis, Heart Diseases, Hypertrophy, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Neoplasm Metastasis are some of the diseases may be linked to Human Matrix metalloproteinase 13, MMP-13 ELISA Kit. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene.