|The Rat MMP1 mmp1 (Catalog #MBS700215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700215 ELISA Kit recognizes Rat (Rattus, General) MMP1.
The MMP1 mmp1 product has the following accession number(s) (GI #283837835) (NCBI Accession #NP_001164610.1) (Uniprot Accession #P13943). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat matrix metalloproteinase 1, MMP-1/Collagenase I ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
Assay Type: Sandwich
Detection Range: 62.5 pg/ml -4000 pg/ml.
Sensitivity: The minimum detectable dose of rat MMP-1 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Rat matrix metalloproteinase 1, MMP-1/Collagenase I ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Fibrosis, Heart Diseases, Inflammation, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasm Invasiveness, Neoplasm Metastasis are some of the diseases may be linked to Rat matrix metalloproteinase 1, MMP-1/Collagenase I ELISA Kit. MMP1 also interacts with the following gene(s): CRP, DDR2, FGF2, HGF, IL1A, IL4, TIMP1, TIMP2, VEGF.