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mIL 20 recombinant protein product blog

Posted on 2014-08-10 08:01:04 by mybiosource_staff
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Tags: Recombinant Protein; Interleukin-20; mIL 20 recombinant protein; mIL 20;
The mIL 20 il20 (Catalog #MBS142256) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.

The mIL 20 il20 product has the following accession number(s) (GI #10946716) (NCBI Accession #NP_067355.1) (Uniprot Accession #Q9JKV9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Ly s-Thr-Leu.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Interleukin-20 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

mIL 20 il20

Solubility: It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard.
Biological Activity: The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/mL of Interleukin-20 recombinant mouse, corresponding to a specific activity of 10,000 units/mg of Interleukin-20 recombinant mouse. The following patways have been known to be associated with this gene. Autoimmune Diseases, Brain Diseases, Fatty Liver, Fibrosis, Immune System Diseases, Inflammation, Lung Diseases, Necrosis, Nervous System Diseases, Pulmonary Fibrosis are some of the diseases may be linked to Recombinant Mouse Interleukin-20. Eye tissues are correlated with this protein.
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