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Medium 199 w/ Earle's w/o L-Glutamine culture media product blog

Posted on 2015-08-24 13:28:39 by mybiosource_staff
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Tags: Culture Media; Medium 199 w/ Earle's w/o L-Glutamine; Medium 199 w/ Earle's w/o L-Glutamine culture media;
The Medium 199 w/ Earle\'s w/o L-Glutamine n/a (Catalog #MBS653449) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

In 1950, Morgan, Morton and Parker described one of the first totally defined media that did not depend largely on animal products or extracts as nutritive sources. This medium supported the growth of primary chick embryo heart cells. It has since become known as Medium 199. Its current use is with the addition of serum in virus and vaccine production, and in the culture of many non-transformed cells. It is also used in combination with less complex media. Medium 199 modified with Earle\'s Balanced Salts is designed for use with cells maintained in ambient (non-CO2) atmospheric conditions. M2852 differs from the original formulation by containing all L-amino acids. The original formulation contained 10 DL-amino acids, which are less biologically active on a per gram basis than the corresponding L-amino acids.

The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution\'s basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle\'s Balanced Salts selection is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Medium 199 w/ Earle\'s w/o L-Glutamine n/a

Endotoxin Level: <= 1EU/ml. pH: 4.9
Solubility: Yellow, clear, complete
Directions per Liter: Dissolve 9.4g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 0.35g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-liable compounds that can be damaged with autoclaving.
Components shown as mg/liter:
Amino Acids: L-Alanine: 25
L-Arginine•HCl: 70
L-Aspartic Acid: 30
L-Cysteine•HCl•H2O: 0.11
L-Cystine•2HCl: 26
L-Glutamic Acid: 75
Glycine: 50
L-Histidine•HCl•H2O: 21.88
Hydroxy-L-Proline: 10
L-Isoleucine: 20
L-Leucine: 60
L-Lysine•HCl: 70
L-Methionine: 15
L-Phenylalanine: 25
L-Proline: 40
L-Serine: 25
L-Threonine: 30
L-Tryptophan: 10
L-Tyrosine•2Na•2H2O: 57.66
L-Valine 25
Inorganic Salts: Calcium Chloride: 200
Ferric Nitrate•9H2O: 0.72
Magnesium Sulfate: 97.7
Potassium Chloride: 400
Sodium Acetate: 50
Sodium Chloride: 6800
Sodium Phosphate Mono: 140
Vitamins: Adenine Sulfate: 10
p-Aminobenzoic Acid: 0.05
L-Ascorbic Acid: 0.05
D-Biotin: 0.01
Choline Chloride: 0.5
Calciferol: 0.1
Folic Acid: 0.01
i-Inositol: 0.05
Menadione, NaBisulfate•3H2O: 0.019
Niacinamide: 0.025
D-Pantothenic Acid•Ca: 0.01
Pyridoxine•HCl: 0.05
Riboflavin: 0.01
Thiamine•HCl: 0.01
DL-a-Tocopherol•PO4•2Na: 0.01
Vitamin A 9Retinol) Acetate: 0.14
Other: 5\'-Adenylic acid: 0.2
Adenosine Triphosphate•2Na: 1
Cholesterol: 0.2
2\'-Deoxy-D-ribose: 0.5
Thymine: 0.3
D-Glucose: 1000
Glutathione: 0.05
Guanine•HCl: 0.3
Hypoxanthine (Na): 0.35
Phenol Red, Sodium: 10
Tween 80: 5
D-Ribose: 0.5
Uracil: 0.3
Xanthine•Na: 0.34
Total: 9.4g/liter.
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