LTA elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; LTA elisa kit; LTA; TNF beta;
The Human LTA lta (Catalog #MBS175776) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175776 ELISA Kit recognizes Human LTA.The LTA lta product has the following accession number(s) (GI #135940) (NCBI Accession #P01374.2) (Uniprot Accession #P01374). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human TNF beta PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Principle of the Assay: The Picokine� Human LTA Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LTA with a 96-well strip plate that is pre-coated with antibody specific for LTA. The detection antibody is a biotinylated antibody specific for LTA. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LTA with immunogen: Expression system for standard: E.coli; Immunogen sequence: L35-L205. The kit is analytically validated with ready-to-use reagents. To measure Human LTA, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LTA in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Human LTA in the sample.
Background/Introduction: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNFbeta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
Samples: Cell culture supernatants, serum and plasma (heparin, edta, citrate)
Detection Range: 62.5 pg/ml - 4,000 pg/ml
Sensitivity: <5 pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human TNF beta PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Heart, Lung, Lymph, Pharynx, Spleen, Thymus, Tonsil tissues are correlated with this protein. LTA also interacts with the following gene(s): LTB, TNFRSF1A. The following patways have been known to be associated with this gene. Coronary Artery Disease, Disease Models, Animal, Gastrointestinal Diseases, Heart Diseases, Inflammation, Lung Diseases, Myocardial Infarction, Necrosis, Sepsis, Thrombosis are some of the diseases may be linked to Human TNF beta PicoKine ELISA Kit.