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LRP1 elisa kit product blog

Posted on 2013-12-18 02:35:14 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; LRP1; LRP1 elisa kit; Low density lipoprotein receptor related protein 1;
The Human LRP1 lrp1 (Catalog #MBS727011) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS727011 ELISA Kit recognizes Human (General) LRP1.

The LRP1 lrp1 product has the following accession number(s) (GI #47940659) (NCBI Accession #AAH72015.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Low density lipoprotein receptor related protein 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) LRP1.

Intended Uses: This LRP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LRP-1.

Principle of the Assay: LRP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LRP-1 antibody and an LRP-1-HRP conjugate. The assay sample and buffer are incubated together with LRP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRP-1 concentration since LRP-1 from samples and LRP-1-HRP conjugate compete for the anti-LRP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LRP-1 from the sample, fewer sites are left to bind LRP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRP-1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 250-5000pg/mL
Sensitivity: 1.0pg/mL. Human Low density lipoprotein receptor related protein 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Atherosclerosis, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Dyslipidemias, Heart Diseases, Inflammation, Liver Diseases, Necrosis, Nervous System Diseases are some of the diseases may be linked to Human Low density lipoprotein receptor related protein 1 ELISA Kit.
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