|The Rat LPS n/a (Catalog #MBS268498) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS268498 ELISA Kit recognizes Rat (Rattus, General) LPS.
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(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Lipopolysaccharides (LPS) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat LPS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant LPS concentration.
Assay Type: Sandwich
Detection Range: 1000 ng/ml-15.6 ng/ml
Sensitivity: Up to 5 ng/ml. Intra-assay Precision: <= 8%
Inter-assay Precision: <= 12%. Rat Lipopolysaccharides (LPS) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.