|The LGALS1 lgals1 (Catalog #MBS232407) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s GALECTIN-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Western Blot (WB).
ELISA: Recombinant human galectin-1 may be used as the standard for ELISA applications with either our purified human galectin-1 antibody or our biotinylated human galectin-1 antibody.
Western Blot: Recombinant human galectin-1 may be used as the positive control for Western Blotting applications with either our purified human galectin-1 antibody or our biotinylated human galectin-1 antibody.
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane. Researchers should empirically determine the suitability of the LGALS1 lgals1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LGALS1 lgals1 product has the following accession number(s) (GI #4504981) (NCBI Accession #NP_002296.1) (Uniprot Accession #P09382). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including GALECTIN-1 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Reconstitution: Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the additiona of 0.1% bovine serum albumin (BSA) is recommended.
Preparation: Purified recombinant galectin-1 expressed in E Coli. Endotoxin Level: < 1 EU/ug
Buffer Solution: 10mM Sodium phosphate pH7.5
Activity: Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obtained at 250 ug/ml.
Target Species: Human. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Squamous Cell, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Liver Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to RECOMBINANT HUMAN GALECTIN-1. LGALS1 also interacts with the following gene(s): BEX2, CD7, GEMIN4, MAX, MYC, POU2AF1, PTPRC, TDG. Blood, Bone, Brain, Embryonic Tissue, Liver, Lung, Muscle, Nerve, Skin, Vascular tissues are correlated with this protein.