|The Leukotactin/Mip-5 n/a (Catalog #MBS570449) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. The Leukotactin/Mip-5 - Control Lysate reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Leukotactin/Mip-5 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Western Blot (WB).
Detects Keratin 5 & 8 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 52.5 kDa band, corresponding to Keratin 8. Researchers should empirically determine the suitability of the Leukotactin/Mip-5 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Leukotactin-1 (Lkn-1), also known as Macrophage inflammatory protein 1 delta (MIP-1), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. Lkn-1, together with mouse C10, mouse MIP-1 and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in Lkn-1 have been shown to form a third disulfide bond. Lkn-1 cDNA encodes a 113 amino acid precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 amino acid mature protein. In recombinant Lkn-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of Lkn-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 amino acids, respectively. The full length and the amino-terminal truncated forms of human Lkn-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR-1 transfected cells. Additionally, Lkn-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors.
Source Note: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation of HeLa cells and mouse myeloma cells.