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Lep recombinant protein product blog

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Posted on 2014-09-13 00:27:45 by mybiosource_staff
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Tags: Leptin; Recombinant Protein; lep; Lep recombinant protein;
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The Lep lep (Catalog #MBS400031) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.

The Lep lep product has the following accession number(s) (GI #6981148) (NCBI Accession #NP_037208.1) (Uniprot Accession #P50596). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids of recombinan t mouse leptin was determined and was found to be Ala-Val-Pr o-Ile-Gln.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including leptin are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.

Lep lep

Solubility: It is recommended to reconstitute the lyophilized recombinant mouse leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Protein Content: Protein quantitation of recombinant mouse leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates: The purified recombinant mouse leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of mouse leptin
Formulation: The recombinan protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity: recombinant mouse leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Lep also interacts with the following gene(s): Ghrl, Il6, Jak2, Lepr, Pparg, Socs3, Stat3. Brain tissues are correlated with this protein. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Fatty Liver, Hyperinsulinism, Inflammation, Insulin Resistance, Liver Diseases, Obesity, Pain, Weight Gain, Weight Loss are some of the diseases may be linked to Mouse leptin.
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