LEP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Leptin; lep; LEP elisa kit;
The Human LEP lep (Catalog #MBS355383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355383 ELISA Kit recognizes Human LEP. MyBioSource\'s Leptin can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of Leptin in human serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the LEP lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LEP lep product has the following accession number(s) (GI #4557715) (NCBI Accession #NP_000221.1) (Uniprot Accession #P41159). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Leptin Human ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Insulin polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated
Background/Introduction: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, An and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
Samples: Rat Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type: Sandwich
Detection Range: 0 uIU/ml-140 uIU/ml
Sensitivity: < 5 uIU/ml.